Ligand Binding Assay Service

As biotherapeutics become more prominent in the drug development space, there is an increasing need for ligand binding assays and biomarker assays to assess therapeutic efficacy. Creative Biolabs provides our honor clients with high-quality immune checkpoint ligand binding assays. Offering experienced insight and seamless execution, our laboratory science team could deliver the data that drives your program from early discovery to the clinic.

Ligand Binding Assays

Immune checkpoints represent a group of membrane proteins expressed on effector cells composed of a variety of co-inhibitory and co-stimulatory pathways. They are involved in eliminating unwanted substances while maintaining their tolerance, which plays a crucial role in immune regulation. Ligand binding assay (LBA) is an effective method used to analyze immune checkpoint proteins. This assay can be used to quantify biotherapeutics and biomarkers and to detect anti-drug antibodies in biological matrices by indirectly measuring the interaction between two molecules or the binding of molecules to antibodies, receptors, and other large complex molecules.

In recent years, LBAs have evolved from enzyme-linked immunosorbent assays (ELISA) and radioimmunoassay (RIA) and to include a wide range of highly sensitive, non-radioactive binding assays. The expanded applications with the required sensitivity, high sample throughput, and minimal sample clean-up have made ligand binding assays an essential technique for biotherapeutic quantitation. LBAs are grouped into four main categories: direct, indirect, sandwich or bridging, and competitive (or blocking) formats. When the detection is based on an enzymatic reaction, the readout is absorbance or fluorescence in the case of ELISA. Creative Biolabs also offers platforms using electrochemiluminescence (ECL), cell-based approaches as alternative detection methods for LBAs to meet your nonclinical program needs.

Services at Creative Biolabs

Creative Biolabs has an extensive knowledge bank of experience and know-how in developing and validating selective, sensitive, and reproducible methods for biotherapeutics. Our immune checkpoint ligand binding assay services include but not limited to the following:

• Non-cell-based ligand-binding assay
• Cell-based binding assay
• Tailored roadmap design according to specific immune checkpoint targets

Ligand binding assays in the area of bioanalysis are not an independent discipline. Thus, scientists at Creative Biolabs work closely with our immunogenicity, pharmacokinetic, and statistical specialists to deliver the appropriate solutions and interpretations applicable to your program. If you are looking for related services, please feel free to contact us for more information.

All listed customized services & products are for research use only, not intended for pharmaceutical, diagnostic, therapeutic, or any in vivo human use.